Hendrie picked up a groin injury in the Carling Cup semi-final 2nd leg against Bolton on Tuesday night while Hitzlsperger pulled a thigh muscle.
Both are doubtful for Villa's trip to The Walkers Stadium on Saturday.
"Hendrie has pulled a groin while Hitzlsperger has pulled his thigh muscle," said manager David O'Leary after the game with Bolton.
"We need to steer clear of injuries not be adding to them with the size of the squad we have."