The Norwegian international is still struggling to overcome the toe infection that kept him out of the side that drew 0-0 with Everton at Villa Park last weekend.
“I’ll know nearer the kick-off, but I’d say it would be highly unlikely he’ll make it," said Villa boss David O'Leary.
If Johnsen fails to make the side for the game then Dion Dublin is expected to deputise at the heart of the Villa defence once again.